Journal: Molecular carcinogenesis

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.

doi: 10.1002/mc.23838

Figure Lengend Snippet: FIGURE 6 | Effects of exogenous Galectin‐9 intervention and/or CD137 overexpression on the CT26 tumor model. (A) Collective photograph of the mice at the end of the experiment. (B) Collective photograph of the completely excised tumor tissues from each group of mice. (C) Growth curves of tumors in each group of mice. (D) Trends in body weight changes for each group of mice. (E) Representative images of the distribution of Ki67 immunohistochemical staining in the tumor tissues of each group. (F) Comparative analysis of the proportion of Ki67 positive cells in the tumor tissues of each group. Data are presented as mean ± SD (n = 6). *p < 0.05 versus vehicle or Gal‐9, #p < 0.05 versus CD137‐OE. SD, standard deviation.

Article Snippet: The mice were divided into the following experimental groups: Gal‐9 Group: Mice were given a daily intravenous injection of 30 μg/mouse of Gal‐9 (MedChemExpress, Monmouth Junction, NJ, USA) [22] after tumor cell inoculation.

Techniques: Over Expression, Immunohistochemical staining, Staining, Standard Deviation

Journal: Molecular carcinogenesis

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.

doi: 10.1002/mc.23838

Figure Lengend Snippet: FIGURE 7 | Effects of Galectin‐9 supplementation and CD137 knockout/overexpression on the MC38 tumor model. (A) Collective photograph of the mice at the end of the experiment. (B) Collective photograph of the completely excised tumor tissues from each group of mice. (C) Growth curves of tumors in each group of mice. (D) Trends in body weight changes for each group of mice. (E) Representative images of the distribution of Ki67 immunohistochemical staining in the tumor tissues of each group. (F) Comparative analysis of the proportion of Ki67 positive cells in the tumor tissues of each group. Data are presented as mean ± SD (n = 6). *p < 0.05 versus vehicle, #p < 0.05 versus Gal‐9, &p < 0.05 versus CD137‐OE, ▲p < 0.05 versus Tnfrsf9‐KO. SD, standard deviation.

Article Snippet: The mice were divided into the following experimental groups: Gal‐9 Group: Mice were given a daily intravenous injection of 30 μg/mouse of Gal‐9 (MedChemExpress, Monmouth Junction, NJ, USA) [22] after tumor cell inoculation.

Techniques: Knock-Out, Over Expression, Immunohistochemical staining, Staining, Standard Deviation

Journal: Molecular carcinogenesis

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.

doi: 10.1002/mc.23838

Figure Lengend Snippet: FIGURE 8 | Investigating the impacts of Galectin‐9 supplementation and CD137 knockout/overexpression on the infiltration of immune cells on the tumor microenvironment of murine colon cancer. (A) Representative images of CD137 expression distribution in CT26 and MC38 colon cancer tumor tissues, demonstrated through immunohistochemical staining. (B) Comparative analysis of the proportion of CD137‐positive cells in CT26 and MC38 colon cancer tumor tissues. (C) Distribution of activated T cells in CT26 colon cancer tumor tissues, shown using CD3 and CD137 dual immunofluorescence labeling. (D, E) Comparative analysis of the proportion of CD3+ cells and CD3+CD137+ positive cells in CT26 colon cancer tumor tissues. (F) Similarly, using CD3 and CD137 dual immunofluorescence labeling, the distribution of activated T cells in MC38 colon cancer tumor tissues are shown. (G, H) Comparative analysis of the proportion of CD3+ and CD3+CD137+ positive cells in MC38 colon cancer tumor tissues. Data are presented as mean ± SD (n = 6). *p < 0.05 versus vehicle, #p < 0.05 versus Gal‐9, &p < 0.05 versus CD137‐OE, ▲p < 0.05 versus Tnfrsf9‐KO. SD, standard deviation.

Article Snippet: The mice were divided into the following experimental groups: Gal‐9 Group: Mice were given a daily intravenous injection of 30 μg/mouse of Gal‐9 (MedChemExpress, Monmouth Junction, NJ, USA) [22] after tumor cell inoculation.

Techniques: Knock-Out, Over Expression, Expressing, Immunohistochemical staining, Staining, Immunofluorescence, Labeling, Standard Deviation

Journal: Molecular carcinogenesis

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.

doi: 10.1002/mc.23838

Figure Lengend Snippet: FIGURE 9 | Effects of Galectin‐9 supplementation and CD137 knockout/overexpression on T cell activation in murine colon cancer tumor tissues. (A) Representative bands of phosphorylated CD3ζ, total CD3ζ, phosphorylated ZAP70, and total ZAP70 protein expression in CT26 tumor tissues were detected using western blot analysis. (B) Quantitative analysis compared the levels of phosphorylated CD3ζ in various groups of CT26 tumor tissues. (C) Quantitative analysis compared the levels of phosphorylated ZAP70 in various groups of CT26 tumor tissues. (D) Representative bands of phosphorylated CD3ζ, total CD3ζ, phosphorylated ZAP70, and total ZAP70 protein expression in MC38 tumor tissues were detected using western blot analysis. (E) Quantitative analysis compared the levels of phosphorylated CD3ζ in various groups of MC38 tumor tissues. (F) Quan- titative analysis compared the levels of phosphorylated ZAP70 in various groups of MC38 tumor tissues. Data are presented as mean ± SD (n = 6). *p < 0.05 versus vehicle, #p < 0.05 versus Gal‐9, &p < 0.05 versus CD137‐OE, ▲p < 0.05 versus Tnfrsf9‐KO. SD, standard deviation.

Article Snippet: The mice were divided into the following experimental groups: Gal‐9 Group: Mice were given a daily intravenous injection of 30 μg/mouse of Gal‐9 (MedChemExpress, Monmouth Junction, NJ, USA) [22] after tumor cell inoculation.

Techniques: Knock-Out, Over Expression, Activation Assay, Expressing, Western Blot, Standard Deviation